Miller M A, Mazewski C M, Yousuf N, Sheikh Y, White L M, Yanik G A, Hyams D M, Lampkin B C, Raza A
Barrett Cancer Center, University of Cincinnati Medical Center, Ohio 45267.
J Histochem Cytochem. 1991 Apr;39(4):407-12. doi: 10.1177/39.4.2005370.
Cell cycle kinetics of solid tumors in the past have been restricted to an in vitro labeling index (LI) measurement. Two thymidine analogues, bromodeoxyuridine (BrdU) and iododeoxyuridine (IUdR), can be used to label S-phase cells in vivo because they can be detected in situ by use of monoclonal antibodies (MAb) against BrdU (Br-3) or IUdR (3D9). Patients with a variety of solid tumors (lymphoma, brain, colon cancers) received sequential intravenous IUdR and BrdU. Tumor tissue removed at the end of infusion was embedded in plastic and treated with MAb Br-3 and 3D9 sequentially, using a modification of a previously described method. Clearly single and double labeled cells were visible, which enabled us to determine the duration of S-phase (Ts) and the total cell cycle time (Tc), in addition to the LI in these tumors. Detailed control experiments using tissue culture cell lines as well as bone marrow cells from leukemic patients are described, including the comparison of this double label technique with our previously described BrdU-tritiated thymidine technique. We conclude that the two methods are comparable and that the IUdR/BrdU method permits rapid and reliable cell cycle measurements in solid tumors.
过去，实体瘤的细胞周期动力学研究仅限于体外标记指数（LI）测量。两种胸苷类似物，溴脱氧尿苷（BrdU）和碘脱氧尿苷（IUdR），可用于体内标记S期细胞，因为它们可通过使用抗BrdU（Br-3）或IUdR（3D9）的单克隆抗体（MAb）进行原位检测。患有多种实体瘤（淋巴瘤、脑癌、结肠癌）的患者接受了连续静脉注射IUdR和BrdU。输注结束时切除的肿瘤组织包埋在塑料中，并使用先前描述方法的改进方法依次用MAb Br-3和3D9处理。清晰可见单标记和双标记细胞，这使我们能够确定这些肿瘤中的S期持续时间（Ts）和总细胞周期时间（Tc）以及LI。描述了使用组织培养细胞系以及白血病患者骨髓细胞进行的详细对照实验，包括将这种双标记技术与我们先前描述的BrdU-氚标记胸苷技术进行比较。我们得出结论，这两种方法具有可比性，并且IUdR/BrdU方法允许在实体瘤中快速且可靠地进行细胞周期测量。
