Ray F A, Kraemer P M
Cellular and Molecular Biology Group, Los Alamos National Laboratory, New Mexico.
Cancer Genet Cytogenet. 1992 Mar;59(1):39-44. doi: 10.1016/0165-4608(92)90155-2.
Nine newly immortal lines of human fibroblasts transfected with SV40 T antigen were examined for recurrent chromosome losses. In order of decreasing frequency, all nine lines had three or more of the following minimal deletions specifically associated with the immortalization event: del(6)(q21), del(3)(p24), del(1)(p34), del(4)(p25), del(5)(p14), del(11)(p11), del(11)(q14), del(12)(p12), and del(14)(p?). Many other chromosome changes were not clearly associated with immortalization, but were acquired during other stages of this multistep model of neoplastic transformation. We propose that these chromosome loci associated with immortalization are candidates for the location of genes involved in cellular senescence.
对九株转染了SV40 T抗原的新的永生人成纤维细胞系进行了反复染色体丢失检测。按频率递减顺序，所有九个细胞系都有三个或更多以下与永生化事件特异性相关的最小缺失：del(6)(q21)、del(3)(p24)、del(1)(p34)、del(4)(p25)、del(5)(p14)、del(11)(p11)、del(11)(q14)、del(12)(p12)和del(14)(p?)。许多其他染色体变化与永生化没有明显关联，而是在这种肿瘤转化多步骤模型的其他阶段获得的。我们提出，这些与永生化相关的染色体位点是参与细胞衰老的基因所在位置的候选者。
