单克隆抗体MON-114：检测人肺癌中神经内分泌分化的标志物

Monoclonal antibody MON-114: detection of a marker for neuroendocrine differentiation in human lung cancer.

作者信息

van Duijnhoven H L, Moors E H, Groeneveld A, Timmer E D, de Leij L F, Wagenaar S S, Polak J M, van de Ven W J

机构信息

Department of Biochemistry, University of Nijmegen, The Netherlands.

出版信息

Cancer Lett. 1992 Mar 31;63(1):61-6. doi: 10.1016/0304-3835(92)90090-i.

DOI:10.1016/0304-3835(92)90090-i

PMID:1313334

Abstract

Mouse monoclonal antibody MON-114 was generated upon immunization with a human small cell lung carcinoma cell line GLC-19. Immunohistochemical analysis of normal tissues with MON-114 showed staining of the adrenal gland, brain and peripheral nerves. With respect to human lung carcinomas, 7 out of 8 small cell lung carcinomas were positively stained as well as 5 out of 5 carcinoid tumors, whereas only 4 out of 31 squamous cell carcinomas and 3 out of 19 adenocarcinomas were weakly stained. Furthermore, 1 large cell carcinoma was negative for MON-114 staining. Apparently, MON-114 stains cells of neuroendocrine differentiation.

摘要

小鼠单克隆抗体MON-114是用人小细胞肺癌细胞系GLC-19免疫制备的。用MON-114对正常组织进行免疫组织化学分析显示，肾上腺、脑和周围神经有染色。在人肺癌方面，8例小细胞肺癌中有7例呈阳性染色，5例类癌肿瘤中有5例呈阳性染色，而31例鳞状细胞癌中只有4例、19例腺癌中只有3例呈弱阳性染色。此外，1例大细胞癌MON-114染色为阴性。显然，MON-114可对神经内分泌分化的细胞进行染色。