Moss F, Bobrow L G, Sheppard M N, Griffiths M, Rowe D, Beverley P C, Addis B, Souhami R L
J Pathol. 1986 Jun;149(2):103-11. doi: 10.1002/path.1711490205.
Seventy-one lung carcinomas from 66 different patients were stained with a panel of monoclonal antibodies. Twenty-nine were small cell lung carcinoma (SCLC), 15 adenocarcinomas, 17 squamous carcinomas and 10 large cell carcinomas. Three of the monoclonal antibodies recognize different cytokeratins, three recognize other epithelial antigens and one recognizes a neural antigen. Both formalin-fixed and cryopreserved tumours were studied using an indirect immunoperoxidase method. 23/29 SCLC reacted with all but one of the antibodies which recognize epithelial antigens. This staining was similar to that seen in non small cell lung carcinomas (NSCLC) and provides further evidence that SCLC are true epithelial tumours. All but one of the SCLC stained with the antibody recognizing a neural antigen. This antibody did not stain squamous or adenocarcinomas. However, four of the large cell carcinomas stained well with this antibody, suggesting that SCLC and some large cell carcinomas share a common pathway of differentiation. There were variations of staining seen both within and between tumours. This has obvious implications if immunotargetting with monoclonal antibodies is to be used diagnostically or therapeutically.
用一组单克隆抗体对66例不同患者的71个肺癌进行染色。其中29个为小细胞肺癌（SCLC），15个为腺癌，17个为鳞癌，10个为大细胞癌。三种单克隆抗体识别不同的细胞角蛋白，三种识别其他上皮抗原，一种识别神经抗原。采用间接免疫过氧化物酶法对福尔马林固定和冷冻保存的肿瘤进行研究。23/29的SCLC与所有识别上皮抗原的抗体（仅一种除外）发生反应。这种染色与非小细胞肺癌（NSCLC）中所见相似，进一步证明SCLC是真正的上皮性肿瘤。除一个SCLC外，所有SCLC均被识别神经抗原的抗体染色。该抗体未对鳞癌或腺癌染色。然而，4个大细胞癌被该抗体良好染色，提示SCLC和一些大细胞癌具有共同的分化途径。肿瘤内部和肿瘤之间均可见染色差异。如果要将单克隆抗体免疫靶向用于诊断或治疗，这具有明显的意义。
