Martijena I D, Salvatierra N A, Arce A
Cátedra de Química Biológica, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Argentina.
J Neural Transm Gen Sect. 1992;87(2):97-104. doi: 10.1007/BF01245011.
In young chicks submitted to acute stress by forced swimming there was a significant increase in the number of the measurable [3H]-flunitrazepam receptors in synaptosomal membranes from forebrain. In addition, low subsolubilizing concentrations of Triton X-100 caused a significant increase in the measurable [3H]-flunitrazepam receptor number in synaptosomal membranes from non-stressed chicks. However, this Triton X-100 stimulatory effect was not observed when tested in synaptosomal membranes from stressed chicks. In all cases the affinity remained unchanged. This result suggest that: (i) acute stress and Triton X-100 induce receptor recruitment by enhancing [3H]-flunitrazepam accessibility to a pool of receptors which is unmeasurable either before stress or in absence of detergent; (ii) neither recruitment types are additive and they involve receptors coming from the same nonmeasurable pool; (iii) stress induces a maximal recruitment of existing benzodiazepine receptors; (iiii) the pool of nonmeasurable receptors represents about a quarter of the total in control chicks. The recruitment at a short time of stress could be interpreted in terms involving internalization; recycling or modulation of receptors but not its biosynthesis or degradation.
在通过强迫游泳施加急性应激的幼雏中，前脑突触体膜中可测量的[3H]-氟硝西泮受体数量显著增加。此外，低浓度的 Triton X-100 可使未受应激的雏鸡突触体膜中可测量的[3H]-氟硝西泮受体数量显著增加。然而，在应激雏鸡的突触体膜中进行测试时，未观察到这种 Triton X-100 的刺激作用。在所有情况下，亲和力均保持不变。该结果表明：(i) 急性应激和 Triton X-100 通过增强[3H]-氟硝西泮与一组受体的可及性来诱导受体募集，这组受体在应激前或无去污剂时是不可测量的；(ii) 两种募集类型均无累加性，且它们涉及来自同一不可测量池的受体；(iii) 应激诱导现有苯二氮䓬受体的最大募集；(iiii) 在对照雏鸡中，不可测量受体池约占总数的四分之一。应激短时间内的募集可以用涉及受体内化、再循环或调节的术语来解释，但不是其生物合成或降解。
