Marín R H, Arce A, Martijena I D
Cátedra de Química Biológica, Facultad de Ciencias Exactas Físicas y Naturales, Universidad Nacional de Córdoba, Argentina.
Neurochem Int. 1996 Apr;28(4):425-9. doi: 10.1016/0197-0186(95)00093-3.
A significant increase in the number of measurable [3H]Ro 5-4864 receptors was found in forebrain membranes of chicks submitted to 15 min of acute swim stress compared to non-stressed chicks. In addition, low subsolubilizing concentrations of Triton X-100 caused a significant increase in the measurable [3H]Ro 5-4864 receptor number in forebrain membranes from non-stressed chicks. However, this increase caused by Triton X-100 was not observed when tested in forebrain membranes from stressed chicks. In all cases the affinity remained unchanged. These results suggest that: (1) acute stress and Triton X-100 induce receptor increase by enhancing [3H]Ro 5-4864 accessibility to a pool of receptors not detected before stress or in the absence of detergent; (2) the pool of non-measured receptors represents about a third of the total in control chicks; (3) the increments are not additive and could involve receptors coming from the same non-measured pool; (4) the receptor increase during a short time of stress could be explained by recruitment of receptors but not by an increase in the receptor protein biosynthesis; (5) stress induces a maximal recruitment of measurable [-3H]Ro 5-4864 receptors.
与未受应激的雏鸡相比，在经受15分钟急性游泳应激的雏鸡前脑细胞膜中，可测量的[3H]Ro 5-4864受体数量显著增加。此外，低增溶浓度的 Triton X-100 可使未受应激雏鸡前脑细胞膜中可测量的[3H]Ro 5-4864受体数量显著增加。然而，在应激雏鸡的前脑细胞膜中进行测试时，未观察到 Triton X-100 引起的这种增加。在所有情况下，亲和力均保持不变。这些结果表明：(1) 急性应激和 Triton X-100 通过增强[3H]Ro 5-4864 与应激前或无去污剂时未检测到的受体池的可及性来诱导受体增加；(2) 未测量的受体池约占对照雏鸡总数的三分之一；(3) 增量不是相加的，可能涉及来自同一未测量池的受体；(4) 短时间应激期间的受体增加可以用受体募集来解释，而不是受体蛋白生物合成的增加；(5) 应激诱导可测量的[-3H]Ro 5-4864 受体的最大募集。
