Ogawa E, Kawakami A, Yagi T, Amaya T, Fujise H, Takahashi R
Department of Pathology II, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
J Vet Med Sci. 1992 Feb;54(1):57-62. doi: 10.1292/jvms.54.57.
Oxidative damage to the membrane in canine erythrocytes with inherited high Na, K-ATPase activity (HK cells) was compared with that in normal canine cells (LK cells). When 30 mM beta-acetylphenylhydrazine (APH) was applied to HK and LK cells, lipid peroxidation and hemoglobin denaturation occurred. Lipid peroxidation determined from malondialdehyde (MDA) formation was significantly lower in HK than in LK cells so far as endogenous glutathione (GSH) concentration was maintained at appropriate levels. With the depletion of GSH, MDA formation was accelerated and difference between HK and LK cells was not significant. Denatured hemoglobin bound to the membrane protein was less in HK than in LK cells. During incubation with APH, osmotic fragility increased markedly in LK cells, while HK cells showed very little change. The amounts of total lipid, total and free cholesterol, glycolipid, phospholipid and fatty acids were essentially the same in both cell types. Fatty acid compositions showed very small differences. The membrane of HK cells thus appear to have greater protection against oxidative damage induced by APH, owing to the presence of excess GSH in HK cells. The capability of HK cells to withstand oxidative damage would not be due to differences in membrane lipid compositions.
将遗传性高钠钾 -ATP 酶活性犬红细胞（HK 细胞）与正常犬红细胞（LK 细胞）的膜氧化损伤进行了比较。当向 HK 细胞和 LK 细胞施加 30 mM 的β - 乙酰苯肼（APH）时，发生了脂质过氧化和血红蛋白变性。只要内源性谷胱甘肽（GSH）浓度维持在适当水平，由丙二醛（MDA）形成所确定的脂质过氧化在 HK 细胞中显著低于 LK 细胞。随着 GSH 的消耗，MDA 形成加速，HK 细胞和 LK 细胞之间的差异不显著。与膜蛋白结合的变性血红蛋白在 HK 细胞中比在 LK 细胞中少。在与 APH 孵育期间，LK 细胞的渗透脆性显著增加，而 HK 细胞变化很小。两种细胞类型中总脂质、总胆固醇和游离胆固醇、糖脂、磷脂和脂肪酸的量基本相同。脂肪酸组成差异非常小。因此，由于 HK 细胞中存在过量的 GSH，HK 细胞的膜似乎对 APH 诱导的氧化损伤具有更大的保护作用。HK 细胞耐受氧化损伤的能力并非由于膜脂质组成的差异。
