Caldini R, Chevanne M, Magnelli L
Istituto di Patologia Generale, Università di Firenze, Italy.
J Cell Physiol. 1992 Apr;151(1):172-9. doi: 10.1002/jcp.1041510122.
Pyridine and adenine nucleotide levels were measured in Friend erythroleukaemia cells (FELC) stimulated to growth and induced to differentiate by hexamethylene bisacetamide (HMBA) and N'-methylnicotinamide (N'-MNAM). A three- to fourfold increase in the NADP(H) was found to parallel cell growth stimulation in both the presence and absence of differentiation inducers. NAD(H) increased about twofold in control and to a minor extent in HMBA-treated FELC but did not vary significantly in N'-MNAM-treated cells. ATP was significantly higher in control cells stimulated to growth than in resting ones, but it did not vary in inducer-treated cells. These data confirm the relationship between high NADP(H) levels and cell resumption to growth; moreover they show that NAD(H) pool reduction and NAD/NADH ratio rise are associated with the process of FELC differentiation. The activities of NAD pyrophosphorylase and NAD kinase are much more enhanced in growth-stimulated FELC than in resting ones. On the other hand transition from the quiescent to the proliferative state was accompanied by a decrease in the activity of poly(ADP-ribose) polymerase. A decrease in poly(ADP-ribose) polymerase activity was also found in differentiated cells in contrast to controls.
在由六亚甲基双乙酰胺（HMBA）和N'-甲基烟酰胺（N'-MNAM）刺激生长并诱导分化的弗瑞德红白血病细胞（FELC）中，测定了吡啶和腺嘌呤核苷酸水平。发现在有无分化诱导剂的情况下，NADP（H）增加三到四倍与细胞生长刺激平行。在对照中，NAD（H）增加约两倍，在HMBA处理的FELC中增加幅度较小，但在N'-MNAM处理的细胞中没有显著变化。被刺激生长的对照细胞中的ATP显著高于静止细胞，但在诱导剂处理的细胞中没有变化。这些数据证实了高NADP（H）水平与细胞恢复生长之间的关系；此外，它们表明NAD（H）池减少和NAD/NADH比值升高与FELC分化过程有关。与静止的FELC相比，生长刺激的FELC中NAD焦磷酸化酶和NAD激酶的活性增强得多。另一方面，从静止状态转变为增殖状态伴随着聚（ADP-核糖）聚合酶活性的降低。与对照相比，在分化细胞中也发现聚（ADP-核糖）聚合酶活性降低。
