Morioka K, Tanaka K, Nokuo T, Ishizawa M, Ono T
Gan. 1979 Feb;70(1):37-46.
Nicotinamide, a specific inhibitor of poly(ADP-ribose) synthetase, was found to be a moderate inducer of hemoglobin synthesis in Friend erythroid leukemia cells (FLC). Therefore, the effect of other inducers, s-ch as dimethyl sulfoxide (DMSO), hexamethylene-bisacetamide (HMBA), and butyrate, on poly(ADP-ribose) synthesis was examined. The extent of poly(ADP-ribose) synthesis in nuclei of FLC treated with DMSO or HMBA began to decrease before many phenotypic changes including hemoglobin production and reached 30--50% of the level of nontreated control when the cells enter the stationary phase. FLC variants unresponsive to HMBA or DMSO did not exhibit as low an activity of poly(ADP-ribose) synthesis as their parent cells did by treatment with these inducers. In contrast, butyrate stimulated poly(ADP-ribose) synthesis transiently but distinctly (about 50%) at an early stage of culture (6--24 hr), but suppressed it at a later stage. Neither the cell growth nor degradation of poly(ADP-ribose) is correlated with the effect of inducers. These results suggest that the level of poly(ADP-ribose) synthesis is correlated with the differentiation of FLC.
烟酰胺是聚（ADP - 核糖）合成酶的一种特异性抑制剂，被发现是弗瑞德红白血病细胞（FLC）中血红蛋白合成的中度诱导剂。因此，研究了其他诱导剂，如二甲基亚砜（DMSO）、六亚甲基双乙酰胺（HMBA）和丁酸盐对聚（ADP - 核糖）合成的影响。用DMSO或HMBA处理的FLC细胞核中聚（ADP - 核糖）合成的程度在包括血红蛋白产生在内的许多表型变化之前就开始下降，当细胞进入静止期时，达到未处理对照水平的30 - 50%。对HMBA或DMSO无反应的FLC变体在用这些诱导剂处理时，其聚（ADP - 核糖）合成活性并不像它们的亲本细胞那样低。相反，丁酸盐在培养早期（6 - 24小时）短暂但明显地刺激聚（ADP - 核糖）合成（约50%），但在后期抑制它。细胞生长和聚（ADP - 核糖）的降解都与诱导剂的作用无关。这些结果表明聚（ADP - 核糖）合成水平与FLC的分化相关。
