Gallati H, Roth M
J Clin Chem Clin Biochem. 1976 Dec;14(12):581-7.
The activity of the acid phosphatase from prostate was increased by 90% by the addition of 150 mmol/l 1-pentanol to the assay mixture. This activation results in an increased turnover of substrate, so that the phosphomonoester is cleaved more rapidly and a correspondingly larger amount of the release organic residue can be detected. The quantity of free phosphate, however, does not correspond to the substrate turnover, because some of the phosphate residue is transferred from the substrate to the 1-pentanol in a transphosphorylation reaction. The influence of the substrate, buffer, pH and of tartrate on the 1-pentanol-activated prostate phosphatase was investigated.
向测定混合物中添加150 mmol/l 1-戊醇后，前列腺酸性磷酸酶的活性提高了90%。这种激活导致底物周转增加，从而使磷酸单酯更快地被裂解，并且可以检测到相应大量释放的有机残基。然而，游离磷酸盐的量与底物周转不对应，因为在转磷酸化反应中，一些磷酸残基从底物转移到了1-戊醇中。研究了底物、缓冲液、pH值和酒石酸盐对1-戊醇激活的前列腺磷酸酶的影响。
