Kaighn M E
Natl Cancer Inst Monogr. 1978 Dec(49):59-60.
Acid phosphatase activity has been measured in various tissues and in extracts of cultured cells. Cells were solubilized with NP40 at neutral pH, and thymolphthalein monophosphate was used as substrate. Reproducible assays were possible with a few as 10(6) cells.
已对各种组织和培养细胞提取物中的酸性磷酸酶活性进行了测定。细胞在中性pH值下用NP40溶解，并使用百里酚酞单磷酸酯作为底物。使用低至10⁶个细胞即可进行可重复的测定。
