各种组织和培养细胞中酸性磷酸酶活性的测定。
Measurement of acid phosphatase activity in various tissues and cultured cells.
作者信息
Kaighn M E
出版信息
Natl Cancer Inst Monogr. 1978 Dec(49):59-60.
PMID:86169
Abstract
Acid phosphatase activity has been measured in various tissues and in extracts of cultured cells. Cells were solubilized with NP40 at neutral pH, and thymolphthalein monophosphate was used as substrate. Reproducible assays were possible with a few as 10(6) cells.
摘要
已对各种组织和培养细胞提取物中的酸性磷酸酶活性进行了测定。细胞在中性pH值下用NP40溶解，并使用百里酚酞单磷酸酯作为底物。使用低至10⁶个细胞即可进行可重复的测定。
相似文献
1
Measurement of acid phosphatase activity in various tissues and cultured cells.
Natl Cancer Inst Monogr. 1978 Dec(49):59-60.
2
Prostate cancer: normal prostate from human and hamster.
Natl Cancer Inst Monogr. 1978 Dec(49):11-6.
3
Immunochemical identification of prostatic epithelial cells in culture.
Invest Urol. 1979 May;16(6):483-5.
4
Measurement of prostatic acid phosphatase in various cell lines.
Natl Cancer Inst Monogr. 1978 Dec(49):55-6.
5
The effect of iodination on the activity and structure of acid phosphomonoesterase from human prostate.
Acta Biochim Pol. 1968;15(4):369-79.
6
[Immunochemical properties of acid phosphatase of the human prostate gland and its adenoma].
Vopr Med Khim. 1969 May-Jun;15(3):243-7.
7
[Activation of acid prostate phosphatase by 1-pentanol (author's transl)].
J Clin Chem Clin Biochem. 1976 Dec;14(12):581-7.
8
Fractionation and purification of prostatic acid phosphatase.
Invest Urol. 1974 Jul;12(1):13-6.
9
[Cancer of the prostate: prostatic acid phosphatases. Biochemical properties and assay].
Bull Cancer. 1985;72(6):519-26.
10
[Pharmacological study of prostate extracts. (IV). Prostatic acid phosphotases from several different species, and the effect of prostate extracts on these enzymes].
Nihon Yakurigaku Zasshi. 1974 Sep;70(5):609-19.
Zotero 插件