PRIGGE R
Bull World Health Organ. 1953;9(6):843-9.
The author points out that determination of the mean antitoxin titre is inadequate for ascertaining fine differences in the activity of diphtheria and tetanus toxoids, but that these differences become clearly recognizable when the relation is established between the antigen dose and the proportion of individuals whose antitoxin titre exceeds a given "threshold titre" or neutralizes a given amount of toxin.Furthermore, the author states that it is not feasible to assay crude and highly purified diphtheria toxoids against the same preparation, and that two reference preparations are therefore required.
作者指出,仅测定平均抗毒素滴度不足以确定白喉类毒素和破伤风类毒素活性的细微差异,但当建立抗原剂量与抗毒素滴度超过给定“临界滴度”或中和给定毒素量的个体比例之间的关系时,这些差异就会清晰可辨。此外,作者指出,用同一制剂检测粗制和高度纯化的白喉类毒素是不可行的,因此需要两种参考制剂。