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Complementation of the cs dis2-11 cell cycle mutant of Schizosaccharomyces pombe by a protein phosphatase from Arabidopsis thaliana.

作者信息

Nitschke K, Fleig U, Schell J, Palme K

机构信息

Max-Planck-Institut für Züchtungsforschung, Köln, FRG.

出版信息

EMBO J. 1992 Apr;11(4):1327-33. doi: 10.1002/j.1460-2075.1992.tb05177.x.

DOI:10.1002/j.1460-2075.1992.tb05177.x
PMID:1314161
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC556581/
Abstract

The activities of type I protein phosphatases play a central role in eukaryotic cell cycle control. Here, we report the cloning and characterization from the flowering plant Arabidopsis thaliana of a cDNA clone named PP1-At which is highly homologous to protein phosphatase 1. The deduced amino acid sequence of PP1-At shows that the PP1-At protein is 318 amino acid residues long and has a molecular weight of 35,298 Da. The PP1-At protein has strong similarity to all other known protein phosphatase type 1 catalytic subunits. Approximately 62% of the amino acids are identical to type 1 protein phosphatases of rabbit, mouse, Saccharomyces cerevisiae and Schizosaccharomyces pombe. RNA blot analysis revealed a single mRNA species of approximately the same size as the cDNA isolated. The PP1-At-encoded mRNA of 1.3 kb is abundant in most vegetative Arabidopsis tissues, with the lowest level of expression in leaves. When transferred to the fission yeast S.pombe, the PP1-At-encoded protein can rescue a semidominant mutant, cold sensitive (cs) dis2-11, which under nonpermissive conditions is unable to complete chromosome disjunction.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/32ff/556581/a89ecd315bcf/emboj00089-0111-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/32ff/556581/50cc68543bad/emboj00089-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/32ff/556581/dbf65eacdb64/emboj00089-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/32ff/556581/f4c8abe4baf9/emboj00089-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/32ff/556581/a89ecd315bcf/emboj00089-0111-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/32ff/556581/50cc68543bad/emboj00089-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/32ff/556581/dbf65eacdb64/emboj00089-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/32ff/556581/f4c8abe4baf9/emboj00089-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/32ff/556581/a89ecd315bcf/emboj00089-0111-a.jpg

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本文引用的文献

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Phosphoprotein Phosphatase of Soybean Hypocotyls: PURIFICATION, PROPERTIES, AND SUBSTRATE SPECIFICITIES .大豆下胚轴磷酸蛋白磷酸酶：纯化、性质和底物特异性。
Plant Physiol. 1980 Sep;66(3):368-74. doi: 10.1104/pp.66.3.368.
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The nuclear envelope lamina is reversibly depolymerized during mitosis.核膜层在有丝分裂期间可逆地解聚。
Cell. 1980 Jan;19(1):277-87. doi: 10.1016/0092-8674(80)90409-2.
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A simple method for displaying the hydropathic character of a protein.一种展示蛋白质亲水性特征的简单方法。
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J Bacteriol. 1998 May;180(10):2616-22. doi: 10.1128/JB.180.10.2616-2622.1998.
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Multiple genes encoding serine/threonine protein phosphatases and their differential expression in Nicotiana tabacum.多个编码丝氨酸/苏氨酸蛋白磷酸酶的基因及其在烟草中的差异表达。
Plant Mol Biol. 1998 Jan;36(2):315-22. doi: 10.1023/a:1005943805988.
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Inward rectifier potassium channels in plants differ from their animal counterparts in response to voltage and channel modulators.植物中的内向整流钾通道在对电压和通道调节剂的反应方面与其动物对应物不同。
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