高等植物核基因中假定的聚腺苷酸化信号：汇编与分析
Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis.
作者信息
Joshi C P
机构信息
Division of Biochemical Sciences, National Chemical Laboratory, Pune, India.
出版信息
Nucleic Acids Res. 1987 Dec 10;15(23):9627-40. doi: 10.1093/nar/15.23.9627.
In animal and viral pre-mRNAS, the process of polyadenylation is mediated through several cis-acting poly (A) signals present upstream and downstream from poly (A) sites. The situation regarding polyadenylation of higher plant pre-mRNAS, however, has remained obscure so far. In this paper, a search for putative poly (A) signals is made by considering the published data from 46 plant genomic DNA sequences. Certain domains in the 3' untranslated regions from nuclear genes of higher plants were compiled and occurrence of sequence motifs such as AATAAA, CAYTG, YGTGTTYY and YAYTG was scored in relation to poly (A) sites. Moreover, consensus sequences for important regions in the 3' untranslated sequences and poly (A) signals were also deduced from the data. It was inferred that sequence motifs similar to poly (A) signals exist around poly (A) sites but some of them are in entirely different spatial relationship than observed in other eukaryotes. This indicates their probable non-involvement in the process of polyadenylation in higher plants necessitating a functional analysis approach to define the plant specific poly (A) signals.
在动物和病毒的前体mRNA中，聚腺苷酸化过程是通过位于聚（A）位点上游和下游的几个顺式作用聚（A）信号介导的。然而，高等植物前体mRNA的聚腺苷酸化情况迄今为止仍不清楚。在本文中，通过考虑来自46个植物基因组DNA序列的已发表数据，对假定的聚（A）信号进行了搜索。对高等植物核基因3'非翻译区的某些结构域进行了汇编，并对诸如AATAAA、CAYTG、YGTGTTYY和YAYTG等序列基序相对于聚（A）位点的出现情况进行了评分。此外，还从数据中推导了3'非翻译序列和聚（A）信号中重要区域的共有序列。据推断，与聚（A）信号相似的序列基序存在于聚（A）位点周围，但其中一些与其他真核生物中观察到的空间关系完全不同。这表明它们可能不参与高等植物的聚腺苷酸化过程，因此需要采用功能分析方法来确定植物特异性聚（A）信号。
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