Zhu Y P, Word R A, Johnston J M
Department of Biochemistry, Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas 75235-9051.
Am J Obstet Gynecol. 1992 Apr;166(4):1222-7. doi: 10.1016/s0002-9378(11)90610-3.
It has recently been suggested that platelet-activating factor is involved in a number of processes associated with reproductive biology. We have demonstrated a critical role for this autacoid in the initiation and maintenance of parturition both in animals and man. In the present investigation, it has been demonstrated that platelet-activating factor affects contraction in strips of human myometrium at concentrations as low as 10(-10) mol/L. A platelet-activating factor receptor has been identified and characterized in human myometrium. Platelet-activating factor acts to cause an increase in intracellular Ca2+ concentration of isolated myometrial smooth muscle cells in culture and an increase in the phosphorylation of the 20 kd light chain of myosin in a concentration-dependent fashion. The presence of platelet-activating factor receptors in human myometrium, the observed increase in Ca2+ concentration and phosphorylation of myosin light chain in the presence of platelet-activating factor provide further support for the importance of this autacoid in the initiation of parturition.
最近有人提出，血小板活化因子参与了许多与生殖生物学相关的过程。我们已经证明，这种自分泌物质在动物和人类分娩的启动和维持过程中起着关键作用。在本研究中，已证明血小板活化因子在低至10(-10)mol/L的浓度下就能影响人子宫肌层条带的收缩。在人子宫肌层中已鉴定并表征了血小板活化因子受体。血小板活化因子的作用是使培养的离体子宫肌层平滑肌细胞内的Ca2+浓度增加，并使肌球蛋白20kd轻链的磷酸化以浓度依赖的方式增加。人子宫肌层中血小板活化因子受体的存在，以及在血小板活化因子存在下观察到的Ca2+浓度增加和肌球蛋白轻链磷酸化，为这种自分泌物质在分娩启动中的重要性提供了进一步的支持。
