Anti-platelet activating factor (PAF) antibody inhibits CFW mouse preimplantation embryo development.

OBJECTIVE

Our purpose was to investigate the effect of anti-PAF antibodies on CFW mouse embryo development in vitro.

DESIGN

We studied the in vitro development of CFW mouse one-cell-stage embryos cultured in MEM supplemented with anti-PAF, anti-IgG, or MEM alone to the hatched blastocyst stage.

RESULTS

Mouse embryos cultured with anti-PAF (1:5 dilution; 61%) significantly decreased embryo development compared to controls (MEM alone; 93%), whereas embryos cultured in anti-mouse IgG-supplemented MEM (1:10 dilution; 93%) had no effect.

CONCLUSIONS

The results provide additional evidence that PAF is produced and secreted by cleavage-stage embryos and is required during the preimplantation period.