使用原位杂交和非放射性探针检测传染性法氏囊病病毒
Detection of infectious bursal disease viruses using in situ hybridization and non-radioactive probes.
作者信息
Jackwood D J, Swayne D E, Fisk R J
机构信息
Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
出版信息
Avian Dis. 1992 Jan-Mar;36(1):154-7.
The in situ hybridization assay was developed for the detection of infectious bursal disease virus (IBDV) infections in chickens. Bursal tissue samples were harvested 4 days following infection with the ST-C, MD, E, IN, or SAL IBDV strain. The cDNA clones STC-243, located on genome segment A, and STC-119, located on genome segment B, were used to prepare non-radioactive probes. Probes were labeled with digoxigenin and detected the homologous ST-C virus and also heterologous viruses in bursal tissue sections. No positive cells were observed in tissue sections from uninfected control chickens.
原位杂交检测法用于检测鸡传染性法氏囊病病毒（IBDV）感染。用ST-C、MD、E、IN或SAL IBDV毒株感染鸡后4天采集法氏囊组织样本。位于基因组A节段的cDNA克隆STC-243和位于基因组B节段的STC-119用于制备非放射性探针。探针用地高辛标记，可检测法氏囊组织切片中的同源ST-C病毒以及异源病毒。在未感染对照鸡的组织切片中未观察到阳性细胞。
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