Hathcock T L, Giambrone J J
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Alabama 36849-5519.
Avian Dis. 1992 Apr-Jun;36(2):206-10.
A cDNA probe was synthesized from the VP-4 region of a virulent field isolate of infectious bursal disease virus (IBDV). The probe was labeled during synthesis with a non-radioactive steroid hapten, digoxigenin. The probe was used to develop a hybridization assay to detect the presence of IBDV in infected cell-culture and tissue suspensions from the bursa of Fabricius of infected chickens. The test was rapid, reproducible, and sensitive, and it could detect four serologic subtypes of IBDV, including the GLS-5 isolate.
从传染性法氏囊病病毒（IBDV）的强毒野外分离株的VP-4区域合成了一个cDNA探针。该探针在合成过程中用非放射性甾体半抗原地高辛进行标记。该探针用于开发一种杂交试验，以检测感染鸡法氏囊中感染细胞培养物和组织悬液中IBDV的存在。该试验快速、可重复且灵敏，能够检测IBDV的四种血清学亚型，包括GLS-5分离株。
