Henderson K S, Jackwood D J
Department of Veterinary Preventive Medicine, Ohio State University, Wooster 44691.
Avian Dis. 1990 Jul-Sep;34(3):744-8.
The use of cDNA probes as diagnostic tools for detecting infectious bursal disease virus (IBDV) antigens was compared with the agar-gel precipitin (AGP) and immunofluorescence (IF) assays. Specific-pathogen-free chickens were inoculated with the STC or IN strains of IBDV in three separate experiments. Tissue samples were collected at various intervals postinoculation (PI) and examined for viral antigens using the IF assay, the AGP assay, and the hybridization assay. Viral antigen was detected by the AGP assay for a period of approximately 3 days beginning 2 days PI and by the IF assay for approximately 4 days beginning 2 days PI. Virus was detected by the hybridization assay through the length of all the experiments (longest experiment was 24 days) beginning 1 day PI. These results suggested that the hybridization assay is more sensitive than the IF assay and the AGP assay.
将作为检测传染性法氏囊病病毒（IBDV）抗原诊断工具的cDNA探针，与琼脂凝胶沉淀试验（AGP）和免疫荧光试验（IF）进行了比较。在三个独立的实验中，给无特定病原体的鸡接种IBDV的STC或IN毒株。在接种后（PI）的不同时间间隔收集组织样本，并使用IF试验、AGP试验和杂交试验检测病毒抗原。AGP试验从接种后2天开始，在大约3天的时间内检测到病毒抗原；IF试验从接种后2天开始，在大约4天的时间内检测到病毒抗原。从接种后1天开始，在所有实验过程（最长实验为24天）中，通过杂交试验均检测到了病毒。这些结果表明，杂交试验比IF试验和AGP试验更灵敏。
