Cunnick J, Takemoto D J
Department of Anatomy and Physiology, Kansas State University, Manhattan 66506.
Biochem Biophys Res Commun. 1992 Apr 15;184(1):461-6. doi: 10.1016/0006-291x(92)91216-d.
Polyclonal antipeptide antisera have been utilized to quantitate the amount of retinal rod outer segment cGMP phosphodiesterase alpha and beta catalytic subunits present in retinas from C57BL/6J mice which are normal or carriers for the rd gene defect. Results suggest that the quantity of PDE-beta subunit is reduced in carrier mice while PDE-alpha and PDE-gamma are not affected. In 21-day-old mice, the PDE-beta was reduced by about one-half while adult carrier mice had even more reduced levels of PDE-beta. Since PDE alpha was not reduced, this suggests that synthesis of PDE alpha and PDE beta may not be coordinately controlled.
多克隆抗肽抗血清已被用于定量测定来自正常或携带rd基因缺陷的C57BL/6J小鼠视网膜中视网膜杆状外段cGMP磷酸二酯酶α和β催化亚基的含量。结果表明，携带基因的小鼠中PDE-β亚基的数量减少，而PDE-α和PDE-γ不受影响。在21日龄的小鼠中，PDE-β减少了约一半，而成年携带基因的小鼠中PDE-β的水平甚至更低。由于PDE-α没有减少，这表明PDE-α和PDE-β的合成可能不是协同控制的。
