牛和牛蛙视网膜视杆外段环磷酸鸟苷磷酸二酯酶的γ亚基对该酶动力学参数的影响。
The effect of the gamma-subunit of the cyclic GMP phosphodiesterase of bovine and frog (Rana catesbiana) retinal rod outer segments on the kinetic parameters of the enzyme.
作者信息
Whalen M M, Bitensky M W, Takemoto D J
机构信息
Life Sciences Division, Los Alamos National Laboratory, NM 87545.
出版信息
Biochem J. 1990 Feb 1;265(3):655-8. doi: 10.1042/bj2650655.
Abstract
Rod-outer-segment cyclic GMP phosphodiesterase (PDE) (subunit composition alpha beta gamma 2) contains catalytic activity in alpha beta. The gamma-subunits are inhibitors. Removal of the gamma-subunits increases Vmax. without affecting the Km. The inhibitory effect of a single gamma-subunit (alpha beta gamma) on the Vmax. of alpha beta is much greater in bovine than in frog (Rana catesbiana) PDE. Bovine PDE in the alpha beta gamma 2 state has a Vmax. that is 2.6 +/- 0.4% of the Vmax. of alpha beta. The removal of one gamma-subunit to give alpha beta gamma results in a Vmax. 5.2 +/- 1% of that for maximal activity. Frog alpha beta gamma 2 has a Vmax. 10.8 +/- 2%, and alpha beta gamma has a Vmax. 50 +/- 18%, of the Vmax. of alpha beta. These data suggest that a single gamma-subunit can inhibit the catalytic activity of active sites on both alpha- and beta-subunits in bovine, but not in frog, rod-outer-segment PDE.
摘要
视杆外段环鸟苷酸磷酸二酯酶（PDE）（亚基组成为αβγ2）的催化活性存在于αβ中。γ亚基起抑制作用。去除γ亚基会增加最大反应速度（Vmax），而不影响米氏常数（Km）。单个γ亚基（αβγ）对αβ的Vmax的抑制作用在牛PDE中比在青蛙（牛蛙）PDE中更强。处于αβγ2状态的牛PDE的Vmax是αβ的Vmax的2.6±0.4%。去除一个γ亚基得到αβγ，其Vmax为最大活性时Vmax的5.2±1%。青蛙的αβγ2的Vmax为αβ的Vmax的10.8±2%，αβγ的Vmax为αβ的Vmax的50±18%。这些数据表明，单个γ亚基可抑制牛视杆外段PDE中α和β亚基上活性位点的催化活性，但对青蛙视杆外段PDE则无此作用。
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