Chytil F
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232.
J Lipid Res. 1992 Mar;33(3):381-4.
In this study, we examined effects of retinol deficiency and three retinoids, all-trans-retinoic acid, 13-cis-retinoic acid, and etretin, the aromatic derivative of retinoic acid on nuclear retinoic acid receptor isoforms alpha, beta, and gamma mRNA in rat adipose tissue. Retinol deficiency caused an almost 50% decrease in isoform beta mRNA levels of adipose tissue, whereas little change occurred in the abundance of alpha and gamma isoforms transcripts in this tissue. Intragastric administration of all-trans-retinoic acid to retinol-deficient rats increased the adipose tissue retinoic acid receptor isoforms beta and gamma mRNA levels sixfold and twofold, respectively, in 4 h as compared to adipose tissue of retinol-deficient rats that were administered cottonseed oil. In contrast to this, 13-cis-retinoic acid and etretin at equimolar doses were not effective in inducing beta or gamma isoforms mRNA in retinol-deficient rats. These results show that adipose tissue isoform beta responds to retinol deficiency, and all-trans-retinoic acid rapidly induces beta and gamma mRNA isoforms in this tissue. Thus, retinoic acid may regulate expression of specific genes through its interaction with retinoic acid receptors in adipocytes.
在本研究中，我们检测了视黄醇缺乏以及三种类视黄醇（全反式维甲酸、13-顺式维甲酸和维甲酸的芳香族衍生物依曲替酯）对大鼠脂肪组织中核视黄酸受体亚型α、β和γ mRNA的影响。视黄醇缺乏导致脂肪组织中β亚型mRNA水平下降近50%，而该组织中α和γ亚型转录本丰度变化不大。与给予棉籽油的视黄醇缺乏大鼠的脂肪组织相比，给视黄醇缺乏的大鼠胃内给予全反式维甲酸4小时后，脂肪组织视黄酸受体亚型β和γ mRNA水平分别增加了6倍和2倍。与此相反，等摩尔剂量的13-顺式维甲酸和依曲替酯对诱导视黄醇缺乏大鼠的β或γ亚型mRNA无效。这些结果表明，脂肪组织β亚型对视黄醇缺乏有反应，全反式维甲酸能迅速诱导该组织中的β和γ mRNA亚型。因此，视黄酸可能通过与脂肪细胞中的视黄酸受体相互作用来调节特定基因的表达。
