全反式维甲酸和13-顺式维甲酸对大鼠肺和肝细胞核维甲酸受体mRNA水平的不同影响。

Differential effects of all-trans and 13-cis-retinoic acid on mRNA levels of nuclear retinoic acid receptors in rat lung and liver.

作者信息

Haq R, Pfahl M, Chytil F

机构信息

Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.

出版信息

Biochem Biophys Res Commun. 1991 Oct 31;180(2):1137-44. doi: 10.1016/s0006-291x(05)81185-4.

DOI:10.1016/s0006-291x(05)81185-4

PMID:1719965

Abstract

The effects of three retinoids, all-trans-retinoic acid (all-trans-RA), 13-cis-RA, and etretin were examined on mRNA abundance of nuclear retinoic acid receptors (RAR-alpha, beta, and gamma) in lung and liver of retinol deficient and chow fed rats. All-trans-RA increased lung RAR-beta mRNA levels 5 or 11-fold in chow fed and retinol deficient rats, respectively. Similarly to lung, liver RAR-beta mRNA levels were 3-fold higher in retinol deficient rats fed all-trans-RA than the rats fed cottonseed oil. Lung RAR-gamma mRNA levels were also induced 2-fold by all-trans-RA. In contrast to this, 13-cis-RA and etretin at equimolar doses failed to enhance lung or liver RAR-beta or lung RAR-gamma mRNA levels in retinol deficient rats. These data for the first time show that all-trans-RA is more effective than its 13-cis-isomer in regulating the expression of RAR-beta and gamma transcripts in adult animal.

摘要

研究了三种维甲酸，即全反式维甲酸（all-trans-RA）、13-顺式维甲酸（13-cis-RA）和依曲替酯，对维生素A缺乏和正常饮食大鼠肺和肝脏中核维甲酸受体（RAR-α、β和γ）mRNA丰度的影响。全反式维甲酸分别使正常饮食和维生素A缺乏大鼠的肺RAR-β mRNA水平提高了5倍或11倍。与肺相似，在喂食全反式维甲酸的维生素A缺乏大鼠中，肝脏RAR-β mRNA水平比喂食棉籽油的大鼠高3倍。全反式维甲酸还使肺RAR-γ mRNA水平升高了2倍。与此相反，等摩尔剂量的13-顺式维甲酸和依曲替酯未能提高维生素A缺乏大鼠的肺或肝脏RAR-β或肺RAR-γ mRNA水平。这些数据首次表明，在调节成年动物中RAR-β和γ转录本的表达方面，全反式维甲酸比其13-顺式异构体更有效。