Sakai T, Satoh K, Narumi K, Abe T, Motomiya M
Department of Internal Medicine, Tohoku University, Sendai, Japan.
Nihon Kyobu Shikkan Gakkai Zasshi. 1992 Mar;30(3):402-6.
Cell-adhesive proteins such as laminin and fibronectin and their specific receptors play an important role in the processes of cancer proliferation, invasion, and metastasis. In the present study, we cloned the cDNA of 67 kDa-laminin receptor both from human lung cultured cell line (IMR90) and from human small cell lung cancer (SBC3), and determined the nucleotide sequences. In addition, the expression of mRNA in 11 lung cancer cell lines with various cell types was estimated by the method of Northern blot hybridization. As a result, 1.2 kb-message was detected in all cancer cell lines examined. It was also demonstrated that the mRNA level of 67 kDa-laminin receptor was inversely proportional to the population doubling time of the cell line (r = -0.80).
层粘连蛋白和纤连蛋白等细胞粘附蛋白及其特异性受体在癌症增殖、侵袭和转移过程中发挥着重要作用。在本研究中，我们从人肺培养细胞系（IMR90）和人小细胞肺癌（SBC3）中克隆了67 kDa层粘连蛋白受体的cDNA，并测定了核苷酸序列。此外，通过Northern印迹杂交法评估了11种不同细胞类型的肺癌细胞系中mRNA的表达。结果显示，在所检测的所有癌细胞系中均检测到1.2 kb的信息。还证明67 kDa层粘连蛋白受体的mRNA水平与细胞系的群体倍增时间成反比（r = -0.80）。
