Satoh K, Narumi K, Sakai T, Abe T, Kikuchi T, Matsushima K, Sindoh S, Motomiya M
Department of Internal Medicine, Tohoku University, Sendai, Japan.
Cancer Lett. 1992 Mar 15;62(3):199-203. doi: 10.1016/0304-3835(92)90096-e.
Cell-adhesive protein laminin and its specific receptor play an important role in the processes of cancer proliferation, invasion and metastasis. In the present study, we cloned the cDNAs of the 67-kDa laminin receptor both from a human lung cell line (IMR90) and from a human lung cancer cell line (SBC3), and determined the nucleotide sequences. In comparison with both cDNA sequences of the protein-coding region, three nucleotide differences were found. These differences in the secondary structure of the protein, however, were caused by nucleotide substitutions. It was also demonstrated that the level of 67-kDa-laminin receptor mRNA was higher in SBC3 than in IMR90.
细胞黏附蛋白层粘连蛋白及其特异性受体在癌症增殖、侵袭和转移过程中发挥重要作用。在本研究中，我们从人肺细胞系（IMR90）和人肺癌细胞系（SBC3）中克隆了67-kDa层粘连蛋白受体的cDNA，并测定了核苷酸序列。与蛋白质编码区的两个cDNA序列相比，发现了三个核苷酸差异。然而，这些蛋白质二级结构的差异是由核苷酸替换引起的。还证明SBC3中67-kDa层粘连蛋白受体mRNA的水平高于IMR90。
