糖修饰的寡核苷酸：含1-(4'-硫代-β-D-呋喃核糖基)-胸腺嘧啶的寡脱氧核苷酸的合成、核酸酶抗性及碱基配对
Sugar modified oligonucleotides: synthesis, nuclease resistance and base pairing of oligodeoxynucleotides containing 1-(4'-thio-beta-D-ribofuranosyl)-thymine.
作者信息
Bellon L, Morvan F, Barascut J L, Imbach J L
机构信息
Université de Montpellier II Sciences, URA 488 du CNRS, France.
出版信息
Biochem Biophys Res Commun. 1992 Apr 30;184(2):797-803. doi: 10.1016/0006-291x(92)90660-d.
PMID:1315535
Abstract
XdT12 and dT5XdT6, where X is 1-(4'-thio-beta-d-ribofuranosyl)-thymine, were synthesized. The first oligonucleotide presents a better stability against calf spleen phosphodiesterase than natural dT12. The second one hybridizes with complementary sequence. However the X:A base pairing stability is lower than natural T:A one.
摘要
合成了XdT12和dT5XdT6，其中X为1-(4'-硫代-β-D-呋喃核糖基)-胸腺嘧啶。与天然的dT12相比，第一种寡核苷酸对小牛脾磷酸二酯酶表现出更好的稳定性。第二种寡核苷酸能与互补序列杂交。然而，X:A碱基对的稳定性低于天然的T:A碱基对。
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