Ozeki T, Hikiji K, Funakoshi K, Tsutsumi M, Yoshida A
3rd Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Int J Exp Pathol. 1992 Feb;73(1):1-8.
The presence of hepatitis C virus sequence was detected in liver tissue extracts by the polymerase chain reaction (PCR) method using primers of non-coding region in six out of eight cases with chronic hepatitis seropositive for Chiron's antibody. Subsequently, liver extracts from these cases were added to cell cultures of Chang cells for 3 days. The liver extracts of the six cases positive for PCR appeared to infect the Chang cells.
采用聚合酶链反应（PCR）方法，使用针对非编码区的引物，在8例慢性肝炎患者中，有6例患者血清学检测Chiron抗体呈阳性，其肝组织提取物中检测到丙型肝炎病毒序列。随后，将这些病例的肝提取物加入Chang细胞培养物中培养3天。PCR检测呈阳性的6例病例的肝提取物似乎感染了Chang细胞。
