通过“巢式”聚合酶链反应检测献血中的丙型肝炎病毒序列及感染性预测

Detection of hepatitis C viral sequences in blood donations by "nested" polymerase chain reaction and prediction of infectivity.

作者信息

Garson J A, Tedder R S, Briggs M, Tuke P, Glazebrook J A, Trute A, Parker D, Barbara J A, Contreras M, Aloysius S

机构信息

Department of Medical Microbiology, University College and Middlesex School of Medicine, London, UK.

出版信息

Lancet. 1990 Jun 16;335(8703):1419-22. doi: 10.1016/0140-6736(90)91446-h.

DOI:10.1016/0140-6736(90)91446-h

PMID:1972209

Abstract

Of 1100 blood donations tested during a prospective study of post-transfusion non-A, non-B hepatitis (NANBH), 6 (0.55%) were repeatedly reactive in a commercial assay for antibodies to the C100 protein of hepatitis C virus. Only 1 of the 6 donations (17%) transmitted NANBH to a recipient. Hepatitis C virus RNA sequences were detected in the serum of the transmitting donor by an assay which used the polymerase chain reaction (PCR) and non-radioisotopic detection. No such sequences were detected in the other 5 donors positive for anti-C100. Stored serum samples from blood donors who had been involved in three episodes of post-transfusion NANBH in 1981 also contained hepatitis C viral sequences. Although the PCR assay in its present form is not suitable for mass donor screening, the presence of hepatitis C viral sequences detected by PCR in blood donations seems a better predictor of infectivity than the presence of anti-C100 alone.

摘要

在一项关于输血后非甲非乙型肝炎（NANBH）的前瞻性研究中，对1100份献血样本进行检测，其中6份（0.55%）在一种检测丙型肝炎病毒C100蛋白抗体的商业检测中呈反复阳性。这6份献血中只有1份（17%）将NANBH传播给了受血者。通过一种使用聚合酶链反应（PCR）和非放射性检测的检测方法，在传播病毒的献血者血清中检测到了丙型肝炎病毒RNA序列。在其他5份抗C100呈阳性的献血者中未检测到此类序列。1981年曾导致3起输血后NANBH事件的献血者的储存血清样本中也含有丙型肝炎病毒序列。虽然目前形式的PCR检测方法不适用于大规模献血者筛查，但在献血样本中通过PCR检测到丙型肝炎病毒序列似乎比仅检测到抗C100更能预测传染性。