Chen B F
Fujian Medical College, Fuzhou.
Zhonghua Zhong Liu Za Zhi. 1992 Nov;13(6):463-6.
AgNOR staining technique was applied to facilitate the pathologic differential diagnosis of 74 breast tumors and 95 non-Hodgkin's lymphoma (NHL). The results showed that the mean AgNOR count per nucleus was significantly different between benign and malignant tumors (P less than 0.01). An AgNOR count per cell less than 3 indicated benign breast lesion and more than 5 implied malignancy. In NHL, AgNOR count that nucleolus less than 4 was indicative of low-grade while more than 6 of high-grade malignancy. In 45 NHL patients followed up, the AgNOR count/cell was high in 28 patients with short survivals than otherwise (17 patients). These observations suggest that the AgNOR could provide useful information about the nucleolus activity in hyperplastic and neoplastic conditions. Electron microscopically, argyrophilic protein was irregular in shape and highly stained with silver in the fibrillar centers and the dense fibrillar component in NHL tumor cells as compared to that seen in normal lymphocytes. This further implies that the NOR Proteins could be used as a marker of ribosomal DNA and its level of transcription is higher in the malignant tumors.
应用AgNOR染色技术辅助74例乳腺肿瘤和95例非霍奇金淋巴瘤（NHL）的病理鉴别诊断。结果显示，良性和恶性肿瘤细胞核的平均AgNOR计数有显著差异（P<0.01）。单个细胞AgNOR计数小于3提示乳腺良性病变，大于5提示恶性。在NHL中，核仁AgNOR计数小于4提示低级别恶性，大于6提示高级别恶性。在45例接受随访的NHL患者中，28例生存期短的患者的AgNOR计数/细胞高于其他患者（17例）。这些观察结果表明，AgNOR可提供有关增生性和肿瘤性疾病中核仁活性的有用信息。电镜观察发现，与正常淋巴细胞相比，NHL肿瘤细胞中嗜银蛋白形状不规则，在纤维中心和致密纤维成分中被银高度染色。这进一步表明，NOR蛋白可作为核糖体DNA的标志物，其转录水平在恶性肿瘤中更高。
