Gan X
Department of Pathology, Guangzhou Municipal Hospital of Oncology.
Chin Med J (Engl). 1995 Feb;108(2):132-7.
The argyrophilic staining technique was used in differential diagnosis between non-Hodgkin's lymphoma (NHL) and reactive hyperplasia (RH) of lymphatic tissues in 30 cases of RH and 71 cases of NHL which were classified as low (11 cases), moderate (49 cases) and high malignant (11 cases) groups. The number and the area of Ag nucleolar organizer region (AgNORs) in 100 cells of each case were examined by both IBAS-200 cytoimage analyser and microscope. The results showed that there was significant difference in the number, but not in area, of AgNOR granules between RH and low malignant group of NHL (P < 0.001). In NHL, the significant differences in the number and area of AgNORs were found between each groups (P < 0.05 or P < 0.001). It was also observed that in NHL, the number of AgNOR was negatively correlated with the area of the granules, and the shapes and distribution patterns of AgNOR were significantly different between each groups. Our results suggest that the quantitative study of AgNOR combining with the histological characteristics is very helpful in the differential diagnosis of RH and NHL and in the classification of NHL.
采用嗜银染色技术对30例淋巴组织反应性增生（RH）和71例非霍奇金淋巴瘤（NHL）进行鉴别诊断，其中NHL分为低恶性组（11例）、中恶性组（49例）和高恶性组（11例）。应用IBAS-200细胞图像分析仪及显微镜检测每组病例100个细胞中嗜银核仁组成区（AgNORs）的数量及面积。结果显示，RH与NHL低恶性组之间AgNOR颗粒数量差异有显著性（P<0.001），面积差异无显著性。在NHL组内，各组之间AgNORs数量及面积差异均有显著性（P<0.05或P<0.001）。同时还观察到，在NHL中，AgNOR数量与颗粒面积呈负相关，各组之间AgNOR的形态及分布模式差异有显著性。结果提示，AgNOR的定量研究结合组织学特征对RH与NHL的鉴别诊断及NHL的分类有很大帮助。
