Harvey A R, Fan Y, Beilharz M W, Grounds M D
Department of Anatomy and Human Biology, University of Western Australia, Nedlands, Perth.
Brain Res Mol Brain Res. 1992 Feb;12(4):339-43. doi: 10.1016/0169-328x(92)90137-z.
A Y-chromosome-specific probe and in situ hybridization technology have been used to monitor the survival and migration of neonatal male glia isografted to the left cerebral hemisphere of adult female mice. More than 95% of the cultured donor glia were glial fibrillary acidic protein (GFAP)-positive astrocytes. By 4 weeks, large numbers of transplanted glia were found in both cerebral hemispheres; the extent of glial migration was greatest in white matter tracts. This method provides a new way of identifying all surviving donor cells within the brains of immunologically compatible hosts.
使用Y染色体特异性探针和原位杂交技术监测移植到成年雌性小鼠左脑半球的新生雄性神经胶质细胞的存活和迁移情况。超过95%的培养供体神经胶质细胞是胶质纤维酸性蛋白（GFAP）阳性星形胶质细胞。到4周时，在两个脑半球均发现大量移植的神经胶质细胞；神经胶质细胞在白质束中的迁移范围最大。该方法为识别免疫相容性宿主脑内所有存活的供体细胞提供了一种新途径。
