Furukawa K, Tengler R, Nakamura M, Urwyler A, de Weck A L, Kanegasaki S, Maly F E
Institute of Clinical Immunology, University of Bern, Switzerland.
Scand J Immunol. 1992 May;35(5):561-7. doi: 10.1111/j.1365-3083.1992.tb03255.x.
Human B lymphocytes express components of the superoxide generating system of phagocytes, NADPH oxidase. We studied regulation of this 'B-cell oxidase' during in vitro blast transformation, using Lucigenin-amplified chemiluminescence (CL) to detect superoxide release. While freshly isolated tonsil B lymphocytes showed no CL responses, culture with phorbol myristate acetate (PMA) and ionomycin induced susceptibility to CL triggering by anti-IgM and anti-HLA-DR. Maximal effects were observed after 3 days of culture with 0.4 ng/ml PMA + 1 microgram/ml ionomycin. Cells from such B lymphoblast cultures showed no CL responses to opsonized zymosan. In contrast, peripheral blood mononuclear cells, where monocytes are the predominant oxidant source, showed CL responses to opsonized zymosan but not to anti-IgM and anti-HLA-DR, either before or after culture with PMA + ionomycin. Culture of B cells with the surface immunoglobulin cross-linking agent staphylococcus aureus Cowan I also led to emergence of a CL, response to anti-IgM, which was enhanced by interferon-gamma. Interestingly, markedly fewer B blasts than freshly isolated B lymphocytes expressed cytochrome b-558 surface antigen. Thus, the B-cell oxidase is up-regulated during blast transformation and can be triggered via surface IgM and HLA-DR; however, this appears to be restricted to a subset of B lymphoblasts.
人类B淋巴细胞表达吞噬细胞超氧化物生成系统的组成成分——NADPH氧化酶。我们利用光泽精增强化学发光法（CL）检测超氧化物释放，研究了体外母细胞转化过程中这种“B细胞氧化酶”的调控情况。新鲜分离的扁桃体B淋巴细胞未表现出CL反应，而用佛波醇肉豆蔻酸酯乙酸酯（PMA）和离子霉素培养可诱导其对由抗IgM和抗HLA - DR引发的CL反应产生敏感性。在含有0.4 ng/ml PMA + 1微克/毫升离子霉素的培养基中培养3天后观察到最大效应。来自此类B淋巴母细胞培养物的细胞对调理酵母聚糖无CL反应。相比之下，外周血单个核细胞（其中单核细胞是主要的氧化剂来源）对调理酵母聚糖有CL反应，但在用PMA +离子霉素培养之前或之后，对抗IgM和抗HLA - DR均无反应。用表面免疫球蛋白交联剂金黄色葡萄球菌Cowan I培养B细胞也会导致出现对抗IgM的CL反应，该反应可被干扰素 - γ增强。有趣的是，表达细胞色素b - 558表面抗原的B淋巴母细胞明显少于新鲜分离的B淋巴细胞。因此，B细胞氧化酶在母细胞转化过程中上调，可通过表面IgM和HLA - DR触发；然而，这似乎仅限于B淋巴母细胞的一个亚群。
