Tengler R S, Furukawa K, de Weck A L, Maly F E
Institute of Clinical Immunology, Inselspital Berne, Switzerland.
J Biolumin Chemilumin. 1993 May-Jun;8(3):159-67. doi: 10.1002/bio.1170080306.
Stimulation of phagocytes by several cytokines causes superoxide generation and consequently chemiluminescence. Since antigen-activated lymphocytes generate cytokines, we investigated whether antigen recognition by mononuclear cells, which contain both lymphocytes and monocytes, is accompanied by changes in lucigenin-dependent chemiluminescence. Mononuclear cells which underwent antigen-induced proliferation showed a delayed rise in lucigenin-dependent chemiluminescence in the absence of other stimuli. The common recall antigen Candida albicans increased spontaneous chemiluminescence of mononuclear cells from unselected donors up to 20-fold over control values after 48-72 h of culture. With Rabies virus vaccine as specific antigenic stimulus, only mononuclear cells from rabies immunized individuals responded with enhanced delayed chemiluminescence. In contrast to opsonized zymosan and phorbol myristate acetate, antigens induced no oxidative burst within one hour after addition. Delayed mononuclear cell chemiluminescence was inhibited by the superoxide scavenger superoxide dismutase and by di-phenylene iodonium, a selective inhibitor of the phagocyte NADPH oxidase. A neutralizing monoclonal antibody against interferon-gamma completely abrogated antigen-induced chemiluminescence. Recombinant interferon-gamma by itself induced delayed mononuclear cell chemiluminescence. Thus, antigen-induced delayed mononuclear cell chemiluminescence represents activation of phagocyte NADPH oxidase by interferon-gamma generated by activated lymphocytes.
几种细胞因子对吞噬细胞的刺激会导致超氧化物生成，进而产生化学发光。由于抗原激活的淋巴细胞会产生细胞因子，我们研究了包含淋巴细胞和单核细胞的单核细胞对抗原的识别是否伴随着鲁米诺依赖性化学发光的变化。经历抗原诱导增殖的单核细胞在没有其他刺激的情况下，鲁米诺依赖性化学发光出现延迟升高。常见的回忆抗原白色念珠菌在培养48 - 72小时后，使未选择供体的单核细胞的自发化学发光比对照值增加了20倍。以狂犬病病毒疫苗作为特异性抗原刺激，只有来自狂犬病免疫个体的单核细胞会出现增强的延迟化学发光反应。与调理后的酵母聚糖和佛波酯不同，抗原在添加后一小时内不会诱导氧化爆发。延迟的单核细胞化学发光受到超氧化物清除剂超氧化物歧化酶和吞噬细胞NADPH氧化酶的选择性抑制剂二亚苯基碘鎓的抑制。一种针对干扰素-γ的中和单克隆抗体完全消除了抗原诱导的化学发光。重组干扰素-γ本身可诱导延迟的单核细胞化学发光。因此，抗原诱导的延迟单核细胞化学发光代表了活化淋巴细胞产生的干扰素-γ对吞噬细胞NADPH氧化酶的激活。
