Ingram D A, Forman M B, Murray J J
Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0111.
Am J Pathol. 1992 May;140(5):1081-7.
Fluosol (Alpha Therapeutic Corporation, Los Angeles, CA) an emulsion of perfluorocarbons with a high oxygen-carrying capacity, was approved as an adjunct to alleviate myocardial ischemia during coronary angioplasty. This drug also significantly enhances myocardial salvage presumably related to an action on the neutrophil. The mechanism by which fluosol and its individual components, including the detergent Pluronic F-68, affected neutrophil function was examined. During the incubation of neutrophils with fluosol, a rapid stimulation of superoxide anion production and degranulation which progressively increased over a 30-minute period was detected. Neutrophils incubated with only Pluronic F-68 produced similar amounts of superoxide anion. Cytochalasin B, an inhibitor of phagocytosis, significantly inhibited this superoxide anion generation. As shown previously, neutrophils incubated with fluosol for 30 minutes and then subsequently stimulated manifested a reduction in lysozyme release as compared with untreated cells. Results of an electron microscopic examination confirmed the cellular uptake of the fluosol within phagocytic vacuoles. Neutrophil viability determined by trypan blue was unaffected after fluosol treatment. These observations show that the fluosol emulsion, primarily through micelles formed by the detergent Pluronic F-68, activates human neutrophils by serving as a phagocytic stimulus, which produces a cell refractory to subsequent stimulation.
氟碳乳剂（加利福尼亚州洛杉矶市的阿尔法治疗公司生产）是一种具有高携氧能力的全氟碳化合物乳剂，已被批准作为冠状动脉血管成形术期间缓解心肌缺血的辅助药物。这种药物还能显著增强心肌挽救作用，推测这与它对中性粒细胞的作用有关。研究了氟碳乳剂及其各个成分，包括去污剂普朗尼克F - 68，影响中性粒细胞功能的机制。在中性粒细胞与氟碳乳剂孵育期间，检测到超氧阴离子产生和脱颗粒迅速受到刺激，且在30分钟内逐渐增强。仅与普朗尼克F - 68孵育的中性粒细胞产生的超氧阴离子量相似。吞噬作用抑制剂细胞松弛素B显著抑制了这种超氧阴离子的产生。如先前所示，与未处理的细胞相比，与氟碳乳剂孵育30分钟然后再受到刺激的中性粒细胞，其溶菌酶释放减少。电子显微镜检查结果证实了氟碳乳剂在吞噬泡内被细胞摄取。用台盼蓝测定的中性粒细胞活力在氟碳乳剂处理后未受影响。这些观察结果表明，氟碳乳剂主要通过去污剂普朗尼克F - 68形成的胶束，作为吞噬刺激物激活人中性粒细胞，从而使细胞对随后的刺激产生不应性。
