Eves E M, Tucker M S, Roback J D, Downen M, Rosner M R, Wainer B H
Department of Pharmacological and Physiological Sciences, University of Chicago, IL 60637.
Proc Natl Acad Sci U S A. 1992 May 15;89(10):4373-7. doi: 10.1073/pnas.89.10.4373.
Clonal cell lines of rat embryonic hippocampal origin have been developed by using retroviral transduction of temperature-sensitive simian virus 40 large tumor antigens. The cell lines undergo morphological differentiation at the nonpermissive temperature and in response to differentiating agents. Immunocytochemical analysis indicates that various lines are derived from progenitors of neuronal, glial, and bipotential lineages. Selected neuronal lines differentiate in response to diffusible factors released by primary glia, and one line of glial lineage supports the maturation of primary neurons in culture. Selected cell lines exhibit different patterns of neurotrophin gene expression that change after differentiation. In some lines, the relative levels of neurotrophin 3 and brain-derived neurotrophic factor message expression may reflect the developmental or regional differential expression seen for these genes in the hippocampus in situ. These hippocampal cell lines, which express markers indicative of commitment to neuronal or glial lineages, are valuable for studies of development and plasticity in these lineages, as well as for studies of the regulation of neural trophic interactions.
通过使用温度敏感型猿猴病毒40大肿瘤抗原的逆转录病毒转导，已建立了源自大鼠胚胎海马的克隆细胞系。这些细胞系在非允许温度下并响应分化因子而发生形态分化。免疫细胞化学分析表明，各种细胞系源自神经元、神经胶质和双能谱系的祖细胞。选定的神经元细胞系响应原代神经胶质细胞释放的可扩散因子而分化，而一个神经胶质谱系的细胞系支持培养中原代神经元的成熟。选定的细胞系表现出不同的神经营养因子基因表达模式，这些模式在分化后会发生变化。在一些细胞系中，神经营养因子3和脑源性神经营养因子信息表达的相对水平可能反映了这些基因在海马原位发育或区域差异表达的情况。这些表达指示神经元或神经胶质谱系特征性标志物的海马细胞系，对于研究这些谱系的发育和可塑性以及神经营养相互作用的调节具有重要价值。
