Gong C
Institute of Medical Biology, Kunming.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1992 Feb;14(1):63-7.
Three lymphoblastoid cell lines (CKM-c, CKM-1 and CKM-8) have been established by EBV-transformation from cord blood. They have been maintained for 1 year after 120 passages with rapid growth. The population doubling times were 24.5h (CKM-1), 30 h (CKM-8) and 36 h (CKM-c). The B lymphocyte features were identified under an electron microscope. EBV antigens including EBNA, VCA-Ig A, MA and EA were negative in all the cell lines. The median chromosome number was 48-50. Interferon (IFN) was induced by NDV treatment of the CKM-c cell line. CKM-8 has been modified to be resistant to both thioguanine (30 micrograms/ml) and Ouabain (10(-5)) for the production of human-human hybridomas.
通过爱泼斯坦-巴尔病毒（EBV）转化脐带血已建立了三株淋巴母细胞系（CKM-c、CKM-1和CKM-8）。经过120代快速生长培养后，它们已维持了1年。群体倍增时间分别为24.5小时（CKM-1）、30小时（CKM-8）和36小时（CKM-c）。在电子显微镜下鉴定出了B淋巴细胞特征。所有细胞系中的EBV抗原，包括EB核抗原（EBNA）、病毒衣壳抗原IgA（VCA-IgA）、膜抗原（MA）和早期抗原（EA）均为阴性。染色体中位数为48 - 50条。用新城疫病毒（NDV）处理CKM-c细胞系可诱导产生干扰素（IFN）。为了制备人-人杂交瘤，CKM-8已被改造为对硫鸟嘌呤（30微克/毫升）和哇巴因（10^(-5)）均具有抗性。
