Rincon J, Prieto J, Patarroyo M
Department of Immunology, Karolinska Institute, Stockholm, Sweden.
Int J Cancer. 1992 May 28;51(3):452-8. doi: 10.1002/ijc.2910510319.
Lymphocytes adhere to cells or extracellular matrices to perform functions relating to cytotoxicity, extravasation and tissue localization, as well as modulation of lymphocyte growth and maturation. This adherence is mainly mediated by 3 families of cell-surface adhesion molecules: integrins, immunoglobulin-related molecules and selectins. Since variations in the degree of adherence may affect the pathophysiology of lymphoproliferative disorders, the expression of a large number of adhesion molecules was analysed on Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines (LCLs), and on EBV-positive or EBV-negative Burkitt's lymphoma (BL) lines, by immunofluorescence flow cytometry and immunoprecipitation with monoclonal antibodies. With regard to the beta 1, beta 2 and beta 3 integrin subfamilies, LCLs strongly expressed CD49d/CD29 (VLA-4), CD11a/CD18 (Leu-CAMa, LFA-1) and CD51/CD61 (vitronectin receptor). These cells also abundantly expressed CD54 (ICAM-1) and CD58 (LFA-3) as well as the "homing receptors" L-selectin (LECAM-1) and CD44. BL lines had considerably lower amounts of VLA-4 than LCLs, and ICAM-1 was expressed only by some of the tumor lines. All other adhesion molecules were absent or minimally expressed in the BL cells.
淋巴细胞粘附于细胞或细胞外基质，以执行与细胞毒性、外渗和组织定位相关的功能，以及调节淋巴细胞的生长和成熟。这种粘附主要由3类细胞表面粘附分子介导：整合素、免疫球蛋白相关分子和选择素。由于粘附程度的变化可能会影响淋巴增殖性疾病的病理生理学，因此通过免疫荧光流式细胞术和单克隆抗体免疫沉淀法，分析了爱泼斯坦-巴尔病毒（EBV）转化的淋巴母细胞系（LCLs）以及EBV阳性或EBV阴性伯基特淋巴瘤（BL）细胞系中大量粘附分子的表达情况。关于β1、β2和β3整合素亚家族，LCLs强烈表达CD49d/CD29（VLA-4）、CD11a/CD18（Leu-CAMa，LFA-1）和CD51/CD61（玻连蛋白受体）。这些细胞还大量表达CD54（ICAM-1）和CD58（LFA-3）以及“归巢受体”L-选择素（LECAM-1）和CD44。BL细胞系中的VLA-4含量比LCLs低得多，并且只有一些肿瘤细胞系表达ICAM-1。所有其他粘附分子在BL细胞中均不存在或表达极少。
