Ferguson M K
Department of Surgery, University of Chicago, Illinois 60637.
J Surg Res. 1992 Apr;52(4):359-63. doi: 10.1016/0022-4804(92)90116-h.
The endothelium regulates smooth muscle tone in blood vessels through the release of endothelium-deprived relaxing factor (EDRF). We hypothesized that the lymphatics possess endothelium-dependent relaxant properties analogous to those in blood vessels. Fresh porcine tracheobronchial lymphatic vessel rings were mounted in organ baths and connected to force-displacement transducers. Rings were submaximally precontracted with 10(-5) M histamine and exposed to cumulative addition of acetylcholine (ACH; 10(-7)-3 x 10(-4) M) or bradykinin (BRD; 10(-8)-3 x 10(-6) M), both of which are known to promote release of EDRF. ACH caused concentration-dependent relaxation (maximum effect = -2.3 +/- 2.6% of initial histamine-induced active tension), while a similar but less pronounced effect was seen with BRD (39.6 +/- 5.4%). The relaxant effects of ACH and BRD were inhibited by collagenase pretreatment and mechanical endothelial denudation. The results confirm our hypothesis that lymphatic vessels possess endothelium-dependent relaxant properties and suggest that lymph vessels can regulate lymph flow through processes similar to those found in blood vessels.
内皮细胞通过释放内皮源性舒张因子（EDRF）来调节血管平滑肌张力。我们推测淋巴管具有与血管类似的内皮依赖性舒张特性。将新鲜猪气管支气管淋巴管环置于器官浴槽中，并连接到力-位移换能器上。用10⁻⁵ M组胺使环产生亚最大预收缩，然后累积加入乙酰胆碱（ACH；10⁻⁷ - 3×10⁻⁴ M）或缓激肽（BRD；10⁻⁸ - 3×10⁻⁶ M），已知这两种物质均可促进EDRF释放。ACH引起浓度依赖性舒张（最大效应 = 初始组胺诱导的主动张力的 -2.3 ± 2.6%），而BRD也有类似但不太明显的效应（39.6 ± 5.4%）。ACH和BRD的舒张效应均被胶原酶预处理和机械性内皮剥脱所抑制。结果证实了我们的假设，即淋巴管具有内皮依赖性舒张特性，并表明淋巴管可通过与血管中类似的过程来调节淋巴流动。
