Reeder L B, DeFilippi V J, Ferguson M K
Department of Surgery, University of Chicago, Illinois 60637, USA.
Am J Physiol. 1996 Dec;271(6 Pt 2):H2501-7. doi: 10.1152/ajpheart.1996.271.6.H2501.
We characterized the responses of lymphatic vascular smooth muscle to histaminergic-receptor stimulation and blockade and explored the mechanisms underlying the histamine-stimulated release of endothelium-derived relaxing factor (EDRF). Fresh porcine tracheobronchial lymph vessel rings mounted in organ baths were stimulated by the cumulative addition of histamine or H1, H2, and H3 receptor-specific agonists in the presence or absence of receptor-specific antagonists. Histamine had a contractile effect on lymphatic vascular smooth muscle that was H1 receptor mediated. No important effects were elicited by H2- or H3-receptor stimulation. Histamine also caused the release of EDRF as demonstrated by an increase in smooth muscle tone in the absence of endothelium and after inhibition of nitric oxide synthase. This effect was strong at high concentrations of histamine and was mediated by H1-receptor stimulation. The results suggest that histamine may contribute to the regulation of lymphatic vascular smooth muscle tone under pathological conditions, an effect that may be controlled through modification of H1-receptor activity.
我们对淋巴管平滑肌对组胺能受体刺激和阻断的反应进行了表征，并探讨了组胺刺激内皮衍生舒张因子（EDRF）释放的潜在机制。将新鲜猪气管支气管淋巴管环安装在器官浴槽中，在存在或不存在受体特异性拮抗剂的情况下，通过累积添加组胺或H1、H2和H3受体特异性激动剂进行刺激。组胺对淋巴管平滑肌有收缩作用，这是由H1受体介导的。H2或H3受体刺激未引发重要影响。组胺还导致EDRF释放，这在无内皮和一氧化氮合酶抑制后平滑肌张力增加中得到证实。这种作用在高浓度组胺时很强，且由H1受体刺激介导。结果表明，组胺可能在病理条件下有助于调节淋巴管平滑肌张力，这种作用可能通过改变H1受体活性来控制。
