Ferguson M K, Williams U E, Leff A R, Mitchell R W
Department of Surgery, University of Chicago, Illinois.
Lymphology. 1993 Sep;26(3):113-9.
We assessed the responsiveness of tracheobronchial lymphatic smooth muscle to mediators of inflammation to determine whether homogeneous responses to histamine and 5-hydroxytryptamine (5-HT) are demonstrated among species typically used in studies of lymph vessels. Fresh porcine and bovine tracheobronchial lymph vessels were suspended from force-displacement transducers in baths containing oxygenated Krebs solution. Concentration-response curves were generated by cumulative addition of histamine (10(-7) to 10(-3) M) or 5-HT (10(-7) to 3 x 10(-4) M). Active tension (AT) was expressed in milligrams and as a percentage of initial vessel ring response to 65mM KCl. Histamine elicited concentration-dependent contraction, yielding maximum AT in porcine rings of 1116 +/- 127 mg (n = 39; 129.1 +/- 10.5% of KCl response) and in bovine rings of 733 +/- 106 mg (n = 20; 65.8 +/- 12.9%; P = 0.0005 for percent responses). PD2 values (negative log10 of the concentration at half-maximum effect) were 4.49 +/- 0.08 and 4.82 +/- 0.08; (P = 0.0034). 5-HT elicited concentration-dependent contraction, yielding maximum AT of 560 +/- 50 mg in porcine rings (n = 15; 97.2 +/- 9.7%) and 2892 +/- 454 mg in bovine rings (n = 27; 159.0 +/- 29%; P < 0.0001 for percent responses). PD2 values were 6.25 +/- 0.05 and 5.28 +/- 0.04 (P < 0.0001). The data demonstrate a role for inflammatory mediators in the modulation of tracheobronchial lymphatic smooth muscle tone that is species- and mediator-specific, and support the potential for paracrine regulation of tracheobronchial lymph flow.
我们评估了气管支气管淋巴管平滑肌对炎症介质的反应性，以确定在淋巴管研究中常用的物种之间是否表现出对组胺和5-羟色胺（5-HT）的同质反应。将新鲜的猪和牛气管支气管淋巴管悬挂在含有充氧克雷布斯溶液的浴槽中的力-位移换能器上。通过累积添加组胺（10^(-7)至10^(-3)M）或5-HT（10^(-7)至3×10^(-4)M）生成浓度-反应曲线。主动张力（AT）以毫克表示，并作为初始血管环对65mM氯化钾反应的百分比。组胺引起浓度依赖性收缩，猪环中的最大AT为1116±127mg（n = 39；氯化钾反应的129.1±10.5%），牛环中的最大AT为733±106mg（n = 20；65.8±12.9%；百分比反应的P = 0.0005）。PD2值（最大效应浓度的负对数10）分别为4.49±0.08和4.82±0.08；（P = 0.0034）。5-HT引起浓度依赖性收缩，猪环中的最大AT为560±50mg（n = 15；97.2±9.7%），牛环中的最大AT为2892±454mg（n = 27；159.0±29%；百分比反应的P < 0.0001）。PD2值分别为6.25±0.05和5.28±0.04（P < 0.0001）。数据表明炎症介质在气管支气管淋巴管平滑肌张力调节中起作用，这种作用具有物种和介质特异性，并支持气管支气管淋巴流旁分泌调节的可能性。
