Barna I
Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest.
Acta Endocrinol (Copenh). 1992 Apr;126(4):350-6. doi: 10.1530/acta.0.1260350.
The effect of various anaesthetics and of the manipulations of the hypothalamo-pituitary-adrenocortical system (hypophysectomy, adrenalectomy and lesion of the mediobasal hypothalamus) was studied on immunoreactive-ACTH levels in the plasma and in the cerebrospinal fluid in the rat. The anaesthetics used (Hypnorm, pentobarbital, urethan and Ketanest) were without effect on immunoreactive-ACTH concentration in the cerebrospinal fluid. Immunoreactive-ACTH was significantly decreased after hypophysectomy and elevated after adrenalectomy in both cerebrospinal fluid and plasma. Destruction of the mediobasal hypothalamus resulted in reduced immunoreactive-ACTH content in the cerebrospinal fluid (about 20% of control) in both experiments, whereas immunoreactive-ACTH levels in the plasma of the lesioned rats were lower only in one of the two experiments performed. These data suggest that the main source of the immunoreactive-ACTH in the cerebrospinal fluid of the rat is the hypothalamus; the contribution of the pituitary gland being less than 50% of the radioimmunoassayable ACTH.
研究了各种麻醉剂以及下丘脑 - 垂体 - 肾上腺皮质系统的操作（垂体切除术、肾上腺切除术和下丘脑中间基底部损伤）对大鼠血浆和脑脊液中免疫反应性促肾上腺皮质激素（ACTH）水平的影响。所用麻醉剂（海洛因、戊巴比妥、乌拉坦和凯他敏）对脑脊液中免疫反应性ACTH浓度无影响。垂体切除术后，脑脊液和血浆中的免疫反应性ACTH显著降低；肾上腺切除术后则升高。在两个实验中，下丘脑中间基底部的损伤均导致脑脊液中免疫反应性ACTH含量降低（约为对照组的20%），而在两个实验中的其中一个实验里，损伤大鼠血浆中的免疫反应性ACTH水平仅有所降低。这些数据表明，大鼠脑脊液中免疫反应性ACTH的主要来源是下丘脑；垂体的贡献小于可通过放射免疫测定的ACTH的50%。
