Lis M, Larivière N, Maurice G, Julesz J, Seidah N, chrétien M
Life Sci. 1982 Apr 5;30(14):1159-64. doi: 10.1016/0024-3205(82)90657-9.
Radioimmunoassay developed to measure N-terminal peptide of pro-opiomelanocortin isolated from porcine pituitaries was used to measure changes in the concentration of immunoreactive material in rat plasma. The N-terminal peptide immunoreactive material decreased in plasma after hypophysectomy of both female and male rats below the level of detectability and substantially increased after adrenalectomy as compared to normal control rats. The same changes were observed when beta-endorphin and ACTH like immunoreactive material was measured. The primary culture of rat anterior pituitary cells released ACTH and N-terminal peptide-like immunoreactive material into the incubation medium. The results seem to indicate that the N-terminal immunoreactive material is a secretory product produced by the pituitary gland.
为测量从猪垂体中分离出的促阿片黑素细胞皮质激素原的N端肽而开发的放射免疫分析法，被用于测定大鼠血浆中免疫反应性物质浓度的变化。雌性和雄性大鼠垂体切除后，血浆中的N端肽免疫反应性物质降至可检测水平以下，与正常对照大鼠相比，肾上腺切除后该物质大幅增加。在测量β-内啡肽和促肾上腺皮质激素样免疫反应性物质时也观察到了相同的变化。大鼠垂体前叶细胞的原代培养将促肾上腺皮质激素和N端肽样免疫反应性物质释放到培养液中。结果似乎表明，N端免疫反应性物质是垂体产生的一种分泌产物。
