McDevitt C A, Muir H
J Bone Joint Surg Br. 1976 Feb;58(1):94-101. doi: 10.1302/0301-620X.58B1.131804.
Biochemical changes in the articular cartilage of the knees of mature dogs, one with natural and four with surgically induced osteoarthritis, have been investigated. The four dogs were killed three, six, nine and forty-eight weeks after division of the right anterior cruciate ligament, the left knees serving as controls. The cartilage of the joints operated on was thicker and more hydrated than the control cartilage; the proteoglycans were more easily extracted and had higher galactosamine/glucosamine molar ratios. The proportion of proteoglycans firmly associated with collagen, and hence not extractable, diminished before fibrillation was demonstrable by indian ink staining of the surface. These biochemical changes were present throughout the entire cartilage of the joints operated on of the dogs killed more than three weeks later, and of the dog with natural osteoarthritis. The results suggest that in response to altered mechanical stresses the chondrocytes synthesise proteoglycans that contain more chondroitin sulphate relative to keratin sulphate than normally, as in immature articular cartilage.
对成年犬膝关节软骨的生化变化进行了研究，其中一只患自然性骨关节炎，四只患手术诱发的骨关节炎。在右侧前交叉韧带切断后3周、6周、9周和48周处死这四只犬，以左侧膝关节作为对照。手术关节的软骨比对照软骨更厚且含水量更高；蛋白聚糖更容易提取，半乳糖胺/葡萄糖胺摩尔比更高。在通过表面印度墨水染色可证明出现原纤维形成之前，与胶原蛋白紧密结合因而不可提取的蛋白聚糖比例降低。这些生化变化在3周后处死的犬的手术关节的整个软骨以及患自然性骨关节炎的犬的软骨中均存在。结果表明，与未成熟关节软骨一样，响应于改变的机械应力，软骨细胞合成的蛋白聚糖相对于硫酸角质素含有更多的硫酸软骨素。
