Liu K, Hu Z, Li H, Prince A M, Inchauspe G
Laboratory of Virology and Parasitology, Lindsley F. Kimball Research Institute, New York Blood Center, New York 10021.
Gene. 1992 May 15;114(2):245-50. doi: 10.1016/0378-1119(92)90582-a.
Hepatitis C virus (HCV) genomic clones were obtained from the serum of Chinese HCV carriers using a polymerase chain reaction-based approach. Consensus sequences were derived from (1) the structural region (nt 1-1543) for one carrier, (2) the hypervariable region V (nt 1156-1233) from four carriers and (3) region V3 from four carriers. Region V3, located in the nonstructural domain NS5 (nt 7066-7137), has been previously shown to be a particularly good marker for the genomic typing of HCV isolates [Inchauspe et al., Proc. Natl. Acad. Sci. USA 88 (1991) 10292-10296]. Comparison of these sequences with sequences from geographically distinct HCV isolates indicates that Chinese HCV strains are closely related to, though distinguishable from, Japanese prototype strains. One amino acid motif, GGAA, located in region V, was found to be conserved only among Chinese isolates. This may define a new subgroup among HCV isolates.
采用基于聚合酶链反应的方法，从中国丙型肝炎病毒（HCV）携带者的血清中获得了HCV基因组克隆。共有序列来自：（1）一名携带者的结构区（核苷酸1 - 1543）；（2）四名携带者的高变区V（核苷酸1156 - 1233）；（3）四名携带者的V3区。V3区位于非结构域NS5（核苷酸7066 - 7137），先前已证明它是HCV分离株基因组分型的一个特别好的标志物[因乔斯普等人，《美国国家科学院院刊》88（1991）10292 - 10296]。将这些序列与来自地理上不同的HCV分离株的序列进行比较表明，中国HCV毒株与日本原型毒株密切相关，尽管可以区分。在V区发现的一个氨基酸基序GGAA仅在中国分离株中保守。这可能定义了HCV分离株中的一个新亚组。
