Yun W S, Ho C S, Panesar N S, Swaminathan R
Prenatal Diagnostic Laboratory, Tsan Yuk Hospital, Hong Kong.
Ann Clin Biochem. 1992 May;29 ( Pt 3):337-42. doi: 10.1177/000456329202900315.
Samples of cord serum from 29 healthy neonates were analysed for digoxin-like immunoreactive substance (DLIS), cortisol, 17 beta-oestradiol, progesterone, dehydroepiandrosterone-sulphate (DHEAS), 17 alpha-hydroxyprogesterone (17OHP), androstenedione, oestriol and ouabain-like activity (OLA; by inhibition of Na+, K+ATPase activity). The mean serum concentration of DLIS was 0.91 (SD = 0.19) nmol/L and the mean OLA was 26.1 (SD = 11.5) nmol/L. There was no correlation between DLIS and OLA. DLIS correlated significantly with oestriol (r = 0.521), progesterone (r = 0.534) and 17OHP (r = 0.43). Stepwise multiple regression analysis showed that 17 beta-oestradiol, progesterone and androstenedione contributed to DLIS and the intercept was 0.64 (SD = 0.127). The concentrations of steroids (17 beta-oestradiol, progesterone, androstenedione) required to displace digoxin by 50% in the digoxin immunoassay and inhibit Na+,K+ATPase in the OLA assay were 10(3)-10(4)-fold higher than those found in cord serum. We conclude that the contribution of these steroids to DLIS is small and that DLIS and OLA measure different compounds.
对29名健康新生儿的脐带血清样本进行了分析，检测其中的地高辛样免疫反应物质（DLIS）、皮质醇、17β-雌二醇、孕酮、硫酸脱氢表雄酮（DHEAS）、17α-羟孕酮（17OHP）、雄烯二酮、雌三醇以及哇巴因样活性（OLA；通过抑制Na +、K + -ATP酶活性进行检测）。DLIS的平均血清浓度为0.91（标准差=0.19）nmol/L，OLA的平均浓度为26.1（标准差=11.5）nmol/L。DLIS与OLA之间无相关性。DLIS与雌三醇（r = 0.521）、孕酮（r = 0.534）和17OHP（r = 0.43）显著相关。逐步多元回归分析表明，17β-雌二醇、孕酮和雄烯二酮对DLIS有影响，截距为0.64（标准差=0.127）。在洋地黄毒苷免疫分析中使洋地黄毒苷置换率达到50%以及在OLA分析中抑制Na +、K + -ATP酶所需的类固醇（17β-雌二醇、孕酮、雄烯二酮）浓度比脐带血清中的浓度高10³ - 10⁴倍。我们得出结论，这些类固醇对DLIS的贡献较小，且DLIS和OLA检测的是不同的化合物。
