Tamura H, Shimoyama S, Sunaga Y, Sakaguchi M, Kuroume T
Department of Pediatrics, Gunma University School of Medicine, Japan.
Angiology. 1992 Oct;43(10):856-65. doi: 10.1177/000331979204301009.
Levels of digoxin-like immunoreactive substance (DLIS) and dehydroepiandrosterone sulfate (DHEA) in urine from patients with mucocutaneous lymph node syndrome (MCLS) were measured by radioimmunoassay. Because DLIS of stored samples was often below the level of detection by conventional immunoassay, the authors used 80% ammonium sulfate and extraction with phosphate buffer and then 80% hot ethanol. To study the origin of raised levels of DLIS in urine, the synthesis of DLIS by cultured human umbilical vein endothelial cells (HUVEC) was tested in vitro. The correlation between DLIS and DHEA levels was not significant. Mean levels of urinary DLIS corrected for creatinine in the patients with MCLS were significantly higher than in both normal and diseased controls. The culture medium of HUVEC was found to contain DLIS activity. These results show that MCLS should be added to the clinical states associated with an increased urinary concentration of DLIS and that the endothelial cells are one source of DLIS in man.
采用放射免疫分析法测定了皮肤黏膜淋巴结综合征（MCLS）患者尿液中地高辛样免疫反应物质（DLIS）和硫酸脱氢表雄酮（DHEA）的水平。由于储存样本的DLIS常低于传统免疫分析法的检测水平，作者采用80%硫酸铵并用磷酸盐缓冲液萃取，然后用80%热乙醇处理。为研究尿液中DLIS水平升高的来源，体外检测了培养的人脐静脉内皮细胞（HUVEC）合成DLIS的情况。DLIS与DHEA水平之间的相关性不显著。MCLS患者经肌酐校正后的尿DLIS平均水平显著高于正常对照和疾病对照。发现HUVEC的培养基含有DLIS活性。这些结果表明，MCLS应被添加到与尿中DLIS浓度增加相关的临床状态中，并且内皮细胞是人类DLIS的一个来源。
