UL42蛋白对单纯疱疹病毒1型编码的DNA聚合酶催化亚基的DNA聚合酶活性的影响。
The effect of the UL42 protein on the DNA polymerase activity of the catalytic subunit of the DNA polymerase encoded by herpes simplex virus type 1.
作者信息
Hart G J, Boehme R E
机构信息
Department of Virology, Glaxo Group Research Ltd., Greenford, Middlesex, UK.
出版信息
FEBS Lett. 1992 Jun 29;305(2):97-100. doi: 10.1016/0014-5793(92)80872-e.
The effect that the UL42 protein of herpes simplex virus type 1 has on the DNA polymerase activity of the DNA polymerase catalytic subunit (Pol) of the same virus has been investigated. The observed effects are critically dependent on the salt used and its concentration, such that the UL42 protein may inhibit, have little or no effect on, or activate the Pol activity, depending on the condition used. The observed effects are due to the values for Km(app) for activated DNA and Vmaxapp for Pol and the Pol-UL42 protein complex differently varying with salt concentration.
对1型单纯疱疹病毒的UL42蛋白对同一病毒的DNA聚合酶催化亚基（Pol）的DNA聚合酶活性的影响进行了研究。观察到的影响严重依赖于所使用的盐及其浓度，以至于根据所采用的条件，UL42蛋白可能抑制、对Pol活性几乎没有影响或激活Pol活性。观察到的影响是由于活化DNA的表观米氏常数（Km(app)）以及Pol和Pol-UL42蛋白复合物的最大反应速度（Vmaxapp）的值随盐浓度的不同而变化。
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