Liu C T, Guo Z M
Department of Clinical and Experimental Physiology, United States Army Medical Research Institute of Infectious Disease, Fort Detrick, Frederick, MD 21702-5011.
Lab Anim Sci. 1992 Jun;42(3):275-9.
The cardiovascular effects of constant intracerebroventricular infusion in anesthetized strain 13 guinea pigs were studied. Bilateral cerebroventricles of the animals were catheterized stereotaxically with two 20-gauge blunt needles, penetrating 5 to 6 mm into the skull. Baseline cerebroventricular pressure values were 1.3 +/- 0.6 mmHg. After artificial cerebrospinal fluid was infused into the left ventricle at 0.5 ml/h, left cerebroventricular pressure increased to 8.1 +/- 1.6 mmHg (P less than 0.01), while right cerebroventricular pressure reached 5.6 +/- 2.2 mmHg within 20 minutes. No significant changes in mean blood pressure or heart rate were observed. When intracerebroventricular infusion rate increased to 5.0 ml/h, cerebrospinal fluid pressures of left and right cerebroventricles increased to 40.0 +/- 4.8 and 38.4 +/- 4.7 mmHg within 10 minutes from baseline values of 1.5 +/- 0.5 and 1.7 +/- 0.7 mmHg, respectively. Simultaneously, mean blood pressure and heart rate increased from 72 +/- 4 to 101 +/- 9 mmHg and from 195 +/- 11 to 218 +/- 17 beats/min, respectively. However, 30 to 50 minutes later, mean blood pressure, heart rate, and cerebrospinal fluid pressure decreased abruptly, and two of four animals died. We suggest that this technique with a low infusion rate (less than 0.5 ml/h) can be used to deliver certain drugs into the brain ventricles directly without producing undesirable effects on blood pressure or heart rate.
研究了在麻醉的13号品系豚鼠中持续脑室内注射的心血管效应。通过立体定位用两根20号钝针将动物的双侧脑室插管，刺入颅骨5至6毫米。基线脑室压力值为1.3±0.6 mmHg。以0.5 ml/h的速度向左侧脑室注入人工脑脊液后，左侧脑室压力升至8.1±1.6 mmHg（P<0.01），而右侧脑室压力在20分钟内达到5.6±2.2 mmHg。平均血压和心率未观察到显著变化。当脑室内注射速度增加到5.0 ml/h时，左侧和右侧脑室的脑脊液压力在10分钟内分别从基线值1.5±0.5和1.7±0.7 mmHg升至40.0±4.8和38.4±4.7 mmHg。同时，平均血压和心率分别从72±4升至101±9 mmHg，从195±11升至218±17次/分钟。然而，30至50分钟后，平均血压、心率和脑脊液压力突然下降，四只动物中有两只死亡。我们认为，这种低注射速度（小于0.5 ml/h）的技术可用于直接将某些药物输送到脑室，而不会对血压或心率产生不良影响。
