Dejong C H, Deutz N E, Soeters P B
Department of Surgery, University of Limburg, Maastricht, The Netherlands.
Lab Anim Sci. 1992 Jun;42(3):280-5.
A new application of an indicator dilution technique, using nonradioactive para-aminohippuric acid, is described for superior sagittal sinus blood flow determination in rats. Superior sagittal sinus blood flow, mainly representing cerebral cortex blood flow, amounted to 541 +/- 49 microliters/min in ketamine-anesthetized, but otherwise normal, rats breathing room air. Increasing arterial pCO2 enhanced superior sagittal sinus blood flow (P less than 0.01), providing evidence that this method correctly measures cerebral blood flow. The respiratory quotient was 1.1 and the cerebral cortex metabolic rate of oxygen consumption was +/- 1.45 mumol/min. Cerebral cortex ammonia uptake was not significantly different from zero and of the amino acid fluxes, only alanine differed from zero (P less than 0.05). Flux measurements are crucial in studies of healthy or altered organ metabolism in both experimental animals and postoperative surgical patients. We devised a simple and economical method of repeated cerebral cortex flux measurement in rats that is a potentially valuable tool in metabolic studies of the cerebral cortex.
本文描述了一种使用非放射性对氨基马尿酸的指示剂稀释技术的新应用，用于测定大鼠上矢状窦血流。上矢状窦血流主要代表大脑皮质血流，在吸入空气、氯胺酮麻醉但其他方面正常的大鼠中，其血流量为541±49微升/分钟。动脉血二氧化碳分压升高可增强上矢状窦血流（P<0.01），这证明该方法可正确测量脑血流量。呼吸商为1.1，大脑皮质氧消耗代谢率为±1.45微摩尔/分钟。大脑皮质氨摄取与零无显著差异，在氨基酸通量中，只有丙氨酸与零不同（P<0.05）。通量测量在实验动物和术后手术患者的健康或改变的器官代谢研究中至关重要。我们设计了一种简单且经济的方法来重复测量大鼠大脑皮质通量，这在大脑皮质代谢研究中是一种潜在有价值的工具。
