ACKERMANN W W, MAASSAB H F
J Exp Med. 1954 Oct 1;100(4):329-39. doi: 10.1084/jem.100.4.329.
An analysis of the growth curve obtained in vitro for influenza virus in chorioallantoic membrane has been made using two known viral inhibitors, methoxinine and alpha-amino-alpha p-methoxyphenylmethanesulfonic acid. The action of these compounds has been shown to be specific for particular phases of viral development. Further, this action is sequential indicating that the inhibited phases do not occur concurrently. The sulfonic acid interferes with the initiation of infection and with the release of newly formed virus from the host-cell. The former effect is dependent upon the relative concentrations of viral inoculum and sulfonic acid. The latter effect is prevented by the action of the receptor-destroying enzyme obtained from cholera vibrio extracts. It is proposed that a function of the viral enzyme is to facilitate the release of virus from the host-cell. The intracellular concentration of virus necessary to initiate this release is increased in the presence of alpha-amino-alpha p-methoxyphenylmethanesulfonic acid. It was found that infection can be initiated in the presence of methoxinine under which condition viral increase is disallowed. The function in viral development of one biochemical process which is inhibited by methoxinine was found to be completed at an interval after initiation of infection and before the appearance of the infectious form of the virus. It is possible to define four stages of viral development in terms of sensitivity to methoxinine and alpha-amino-alpha-p-methoxyphenylmethanesulfonic acid.
利用两种已知的病毒抑制剂——甲硫氨酸和α-氨基-α-对甲氧基苯基甲磺酸，对流感病毒在绒毛尿囊膜上的体外生长曲线进行了分析。已证明这些化合物的作用对病毒发育的特定阶段具有特异性。此外，这种作用是相继发生的，表明被抑制的阶段并非同时出现。磺酸会干扰感染的起始以及新形成的病毒从宿主细胞的释放。前一种效应取决于病毒接种物和磺酸的相对浓度。后一种效应可通过霍乱弧菌提取物中获得的受体破坏酶的作用来阻止。有人提出病毒酶的一个功能是促进病毒从宿主细胞的释放。在α-氨基-α-对甲氧基苯基甲磺酸存在的情况下，启动这种释放所需的细胞内病毒浓度会增加。已发现，在甲硫氨酸存在的情况下可以启动感染，但在此条件下病毒的增殖不被允许。已发现，被甲硫氨酸抑制的一个生化过程在病毒发育中的功能在感染起始后且在病毒感染形式出现之前的一段时间内完成。根据对甲硫氨酸和α-氨基-α-对甲氧基苯基甲磺酸的敏感性，可以确定病毒发育的四个阶段。
